AI Article Synopsis
- Biomembrane coating technologies enable the creation of natural biointerfaces on synthetic nanomaterials, providing a way to mimic cellular components.
- A new one-step method uses centrifugation to non-destructively coat nanoparticles with living cell membranes, preserving their integrity and allowing for the analysis of the membrane proteins.
- The coated nanoparticles showed reduced phagocytosis by certain immune cells while increasing uptake in cancer cells, indicating potential for enhanced medical and pharmaceutical uses.
Article Abstract
Biomembrane coating technologies have been developed to equip synthetic nanomaterials with natural biointerfaces. We report a one-step method for nondestructively coating the biomembranes of "living" cells onto nanoparticle surfaces. By using simple centrifugation, nanoparticles pass through a concentrated layer of living cells. This process mimics exosome release via endocytosis and exocytosis, preserving the membrane integrity of the source cells. The resulting silica nanoparticles were efficiently coated with membrane components from Raw264.7 cells. Nanoflow-liquid chromatography-tandem mass spectrometry confirmed that the proteins composing the membrane originated from the source cells. Additionally, the biomembrane coating suppressed the phagocytosis of silica nanoparticles by Raw264.7 cells while enhancing their uptake by HeLa cells. Our simple and efficient method for living biomembrane coating holds promise for the development of nanoparticles for medical and pharmaceutical applications.
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| http://dx.doi.org/10.1021/acs.nanolett.4c03293 | DOI Listing |
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