AI Article Synopsis
- This study introduces a new method for detecting nucleic acids without labels, utilizing isothermal amplification and specially designed aptamer probes.
- The assay transforms double-stranded DNA into single-stranded targets, which allows for precise hybridization with split dapoxyl aptamer probes.
- This technique achieves extremely high sensitivity (attomolar level) and specificity for detecting changes at the single-base level.
Article Abstract
We report a novel platform for label-free nucleic acid detection using isothermal amplification and light-up aptamer probes. This assay converts double-stranded amplicons into single-stranded targets to enable sequence-specific hybridization with split dapoxyl aptamer probes, offering attomolar sensitivity and single-base specificity.
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| http://dx.doi.org/10.1039/d4nr01638f | DOI Listing |
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