AI Article Synopsis
- The study focuses on identifying oxidative stress-related biomarkers in metabolic dysfunction-associated fatty liver disease (MAFLD) and exploring their mechanisms.
- Through analysis of datasets and network methods like WGCNA, the research identified 59 oxidative stress-related differentially expressed genes (DEGs) and 100 hub genes, with 16 being crucial for MAFLD development.
- A mendelian randomization analysis indicates a causal relationship between the hub gene C-reactive protein (CRP) and MAFLD incidence, suggesting new insights into how oxidative stress impacts this liver disease.
Article Abstract
Metabolic dysfunction-associated fatty liver disease (MAFLD) shows accelerated development under the impact of oxidative stress (OS). There is an imperative to identify OS-related biomarkers in MAFLD and explore their potential mechanistic insights. The objective of this study was to identify OS-related biomarkers in MAFLD and explore their potential mechanisms. DEG analysis was performed using GSE17470 and GSE24807 datasets. An investigative exploration utilizing WGCNA was executed to elucidate hub OS-related genes. The intersection of OS-related hub genes identified by WGCNA and DEGs was systematically employed for thorough analyses. A mendelian randomization (MR) study examined the causal effect of C-reactive protein (CRP) on MAFLD. 59 OS-related DEGs were identified in MAFLD. WGCNA revealed 100 OS-related hub genes in MAFLD. Sixteen OS-related genes have been delineated as critical components in MAFLD. Enrichment analyses, employing GO and KEGG pathways, revealed pathways enriched with these genes. Following PPI analyses, the highest-ranking ten hub genes demonstrating abnormal expression were determined. Ultimately, a two-sample MR analysis demonstrated a causal link between the hub gene CRP and the occurrence of MAFLD. In this study, we harnessed WGCNA to formulate a co-expression network and identified hub OS-related DEGs in MAFLD. Additionally, the hub gene CRP exhibited a significant correlation with the predisposition to MAFLD. These findings offer innovative perspectives on the applications of OS-associated genes in individuals afflicted with MAFLD.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456910 | PMC |
| http://dx.doi.org/10.1016/j.bbrep.2024.101829 | DOI Listing |
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