AI Article Synopsis
- The study investigates how benzyl isothiocyanate (BITC) from cruciferous vegetables affects breast cancer stem-like cells (bCSC), emphasizing its inhibition effects.
- BITC treatment led to increased expression of the microRNA miR-124-3p, which plays a significant role in regulating bCSC, although it doesn't impact cell migration or proliferation.
- The research highlights that miR-124-3p directly interacts with FoxQ1, a protein linked to bCSC, indicating its crucial role in the inhibition process elicited by BITC.
Article Abstract
Purpose: We have shown previously that benzyl isothiocyanate (BITC) derived from cruciferous vegetables inhibits self-renewal of breast cancer stem-like cells (bCSC). The current study provides insights into the mechanism of bCSC inhibition by BITC.
Methods: Quantitative real time-polymerase chain reaction and western blot analysis were performed to detect microRNAs (miRNAs) and Forkhead box Q1 (FoxQ1) protein expression, respectively. The bCSC were characterized by aldehyde dehydrogenase 1 activity and flow cytometric analysis of CD49f /CD133 fraction.
Results: BITC treatment resulted in induction of miR-124-3p expression in MDA-MB-231 and MCF-7 cells. miR-124-3p did not affect BITC-mediated inhibition of cell migration or cell proliferation but it significantly regulated bCSC in response to BITC. We also found that miR-124-3p directly targets the 3'untranslated regions (UTR) of FoxQ1 and negatively regulates its expression. The BITC-mediated inhibition of bCSC was partially attenuated by miR-124-3p inhibitor.
Conclusions: These findings indicate that miR-124-3p plays an important role in BITC-mediated inhibition of bCSC.
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Article Synopsis
- The study investigates how benzyl isothiocyanate (BITC) from cruciferous vegetables affects breast cancer stem-like cells (bCSC), emphasizing its inhibition effects.
- BITC treatment led to increased expression of the microRNA miR-124-3p, which plays a significant role in regulating bCSC, although it doesn't impact cell migration or proliferation.
- The research highlights that miR-124-3p directly interacts with FoxQ1, a protein linked to bCSC, indicating its crucial role in the inhibition process elicited by BITC.
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