Due to the extended political, cultural and economic relations with tropical countries a good knowledge of tropical diseases is required. Since the direct parasitological diagnosis is superior to the serological one it seems logical to improve the culture conditions for laboratory diagnosis and for immunological and chemotherapeutical tests. Trypanosoma cruzi (Chagas) and Leishmania donovani (kala-azar) were tested in vitro. Enlargement of the culture surface at the same volume of the medium at 22 degrees C yielded an increase in the number of protozoa by 100%. When blood of different animal species was added to the medium, rabbit blood and that of other rodents such as rat and mouse showed the highest rate of growth. By using the newly developed semi-defined medium 4 as overlay and as monophasic medium high rates of growth were obtained similar to those obtained with known complex media. Therefore, no further import of relevant substances is necessary.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A ten-year retrospective analysis of risk factors and comorbidities prevalence in a group of septic arthritis patients.
Cent Eur J Public Health
December 2024
Department of Orthopaedics and Traumatology of Locomotory Apparatus, Faculty of Medicine, Pavol Jozef Safarik University and Louis Pasteur University Hospital in Kosice, Kosice, Slovak Republic.
Objectives: The aim of this study was the evaluation of a group of patients treated at the Department of Orthopaedics and Traumatology of Locomotory Apparatus at Luis Pasteur University Hospital in Košice for septic arthritis in relation to risk factors and chronic diseases and its microbial aetiologic profile.
Methods: We conducted a retrospective study of patients including all episodes of septic arthritis from March 2013 to August 2022. The occurrence of chronic diseases, risk factors and its microbiological profile were investigated.
Wall shear stress modulates metabolic pathways in endothelial cells.
Metabolomics
January 2025
Laboratory of Applied Mass Spectrometry, Department of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium.
Introduction: Hemodynamic forces play a crucial role in modulating endothelial cell (EC) behavior, significantly influencing blood vessel responses. While traditional in vitro studies often explore ECs under static conditions, ECs are exposed to various hemodynamic forces in vivo. This study investigates how wall shear stress (WSS) influences EC metabolism, focusing on the interplay between WSS and key metabolic pathways.
View Article and Find Full Text PDFOptical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations.
Bull Environ Contam Toxicol
January 2025
Centro de Estudos do Mar, Universidade Federal do Paraná (UFPR), Pontal do Paraná, PR, Brasil.
Microalgae are often used in different industrial sectors and can be used as indicators of aquatic environmental health. An essential step for cultivating microalgae is assessing the cell density, which is traditionally performed through cell counting by optical microscopy (OM). However, this method has limitations, mainly in terms of runtime and low reproducibility.
View Article and Find Full Text PDFBiomaterial-based strategies for primary human corneal endothelial cells for therapeutic applications: from cell expansion to transplantable carrier.
Biomater Sci
January 2025
Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.
The treatment of corneal blindness due to corneal diseases and injuries often requires the transplantation of healthy cadaveric corneal endothelial graft tissue to restore corneal clarity and visual function. However, the limited availability of donor corneas poses a significant challenge in meeting the demand for corneal transplantation. As a result, there is a growing interest in developing strategies alleviate this unmet need, and one of the postulated approaches is to isolate and expand primary human corneal endothelial cells (HCECs) for use in cell therapy.
View Article and Find Full Text PDFDevelopment of Children's Perceived Nursing Image Scale: A Validity and Reliability Study.
J Eval Clin Pract
February 2025
Dokuz Eylul University, Faculty of Nursing, Pediatric Nursing Department, İzmir, Turkey.
Aim: Professional knowledge and awareness of children begins to create at the age of 3-5, and the professional knowledge have acquired at this age was effective in their future career decision-making. This study aimed to develop the Children's Perceived Nursing Image Scale (CPNIS) and evaluate its psychometric properties.
Methods: The methodological study was carried out in a cross-sectional, descriptive, and correlational design.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!