The development of xylem embolism in 1-year-old stems of Japanese black pine () seedlings was monitored by compact magnetic resonance imaging (MRI) after inoculation with the pinewood nematode (). In parallel, the nematode distribution and population structure in the stems were examined by isolating the nematodes using the Baermann funnel technique. The vertical length and volume of massive embolisms in each seedling were strongly correlated with the maximum relative embolized area (REA) in stem cross-sections. Embolism development and nematode reproduction were not restricted to the inoculation site, as any portion of the stem could be the initial point of a population burst. The nematode population in the stem xylem was strongly correlated with the REA and with the circumferential proportion of cambial death in cross-sections monitored by MRI. The proportion of second-stage juveniles was also correlated with the REA in the xylem. In contrast, the nematode population in bark tissue was not correlated with either the REA or cambial death. These results suggested that nematode reproduction in the cambial zone is the key step in pine wilt disease, and second-stage juveniles were suggested to induce massive embolisms in the advanced stage of the disease.
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http://dx.doi.org/10.1094/PHYTO-06-24-0191-R | DOI Listing |
medRxiv
November 2024
Wisconsin Alzheimer's Disease Research Center, University of Wisconsin School of Medicine and Public Health, 600 Highland Avenue, Madison, WI, 53792, USA.
Introduction: Targeted proteomic assays may be useful for diagnosing and staging Alzheimer's disease and related dementias (ADRD). We evaluated the performance of a 120-marker central nervous system (CNS) NUcleic acid-Linked Immuno-Sandwich Assay (NULISA) panel in samples spanning the AD spectrum.
Methods: Cross-sectional plasma samples (n=252) were analyzed using Alamar's NULISAseq CNS panel.
BMC Psychiatry
December 2024
Department of Psychological Medicine, University of Nigeria, Enugu, Nigeria.
J Clin Med
November 2024
Thoracic Surgery Unit, Cardiovascular and Thoracic Department, University and Hospital Trust-Ospedale Borgo Trento, 37126 Verona, Italy.
: Division of the pulmonary ligament is standard in lower lobectomies, but its application in upper lobectomies remains controversial due to potential complications like atelectasis and bronchial kinking. This retrospective matched cohort study aimed to evaluate the efficacy and safety of ligament resection in upper lobectomies for oncological purposes. : From January 2015 to December 2020, 988 patients who underwent minimally invasive upper lobectomies across multiple centers were identified.
View Article and Find Full Text PDFBrain Sci
October 2024
Center for Work-Related Stress and Occupational Mental Disorders, Azienda Ospedaliero-Universitaria Pisana, 56124 Pisa, Italy.
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