AI Article Synopsis
- A new method for attaching cysteine to molecules using -quinone methides (-QMs) has been developed, showing high specificity for the cysteine amino acid.
- This method demonstrates fast reaction rates, with rate constants reaching 1.67 × 10 M·s.
- Additionally, this conjugation allows for the addition of a fluorescent probe to a HER2 nanobody, enabling targeted labeling of HER2-positive SK-BR-3 cancer cells.
Article Abstract
An efficient and selective method for cysteine conjugation utilizing -quinone methides (-QMs) was developed. -QM labeling exhibits high specificity toward the cysteine residue, as evidenced by its reactivity with various amino acid derivatives, peptides, and proteins. Notably, the -QM-cysteine reactions display robust kinetics with rate constants up to 1.67 × 10 M·s. Furthermore, -QM conjugation enables us to attach a fluorescent probe to a HER2 nanobody, resulting in selective labeling of HER2-positive SK-BR-3 cells.
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| http://dx.doi.org/10.1021/acs.orglett.4c03452 | DOI Listing |
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