Next generation preservation solution using synthetic enzymes added to polyhemoglobin to protect warm ischemic human hepatocytes and cardiomyocytes.

Sci Rep

Artificial Cells and Organs Research Centre, Departments of Phsyiology, Medicine and Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada.

Published: October 2024

This study investigates the potential improvement of polyhemoglobin's protective properties by the addition of 3 synthetic enzymes (neo-carbonic anhydrase, neo-catalase and neo-superoxide dismutase) to polyhemoglobin after 90 and 180 min of warm in-vitro ischemia (100% Nitrogen at 37 °C). Following the warm ischemic shock, cell cultures were subjected to various treatment solutions: Controls; PolyHb; 3 neoenzymes; PolyHb + 3 neoenzymes; PolyHb + 2 neoenzymes. The cultures were then incubated (Oxygen, 5% CO at 37 °C) for 24 h followed by several analyses. Compared to polyhemoglobin alone, this novel solution containing polyhemoglobin + 3 neoeznymes significantly increased the viability, cell growth, albumin production, protection against oxidative stress and cellular injury of human hepatocytes. Moreover, this also protects the viability of human cardiomyocytes. These findings suggest that it could be useful as a pre-transplant cell/organ preservation solution which, in the long-term, could contribute to the development of blood substitutes.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452660PMC
http://dx.doi.org/10.1038/s41598-024-73862-2DOI Listing

Publication Analysis

Top Keywords

preservation solution
8
synthetic enzymes
8
warm ischemic
8
human hepatocytes
8
generation preservation
4
solution synthetic
4
enzymes polyhemoglobin
4
polyhemoglobin protect
4
protect warm
4
ischemic human
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!