AI Article Synopsis
- Golden Gate Assembly (GGA) is an efficient technique for combining multiple DNA fragments, useful for creating complex genetic constructs in metabolic engineering.
- The article details how GGA can be specifically applied to develop and optimize the biosynthesis pathway of lycopene through the assembly of various transcriptional units.
- Additionally, the text discusses methods for characterizing and enhancing the production of lycopene in a synthetic yeast environment.
Article Abstract
Golden Gate Assembly (GGA) represents a versatile method for assembling multiple DNA fragments into a single molecule, which is widely used in rapid construction of complex expression cassettes for metabolic engineering. Here we describe the GGA method for facile construction and optimization of lycopene biosynthesis pathway by the combinatorial assembly of different transcriptional units (TUs). Furthermore, we report the method for characterizing and improving lycopene production in the synthetic yeast chassis.
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| http://dx.doi.org/10.1007/978-1-0716-4220-7_23 | DOI Listing |
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