Fabrication of highly flexible biopolymeric chitosan/agarose based bioscaffold with Matricaria recutita herbal extract for antimicrobial wound dressing applications.

A flexible biopolymer-based antimicrobial wound dressing has the potential to alleviate the burden of bacterial infections in wounds by enhancing antimicrobial effectiveness and promoting faster wound healing. This study focuses on the development of a highly flexible chitosan-agarose (CS-AG) bioscaffold, incorporating Matricaria recutita chamomile flower extract (CH) through a conventional casting method. The flexible CS-AG bioscaffold's physiochemical properties were confirmed by FTIR, indicating secondary interactions, and XRD, showing its crystalline structure. The addition of CH to the optimized CS-AG bioscaffold resulted in significant tensile strength (17.28 ± 0.33 MPa), distinctive structural morphology (SEM), surface roughness (AFM), contact angle, improved thermal properties (DSC), and enhanced thermal stability (TGA). Furthermore, the CH-infused bioscaffold significantly increased swelling capacity (~81.09 ± 1.74 % over 48 h), and degradation profile (~52 % over 180 h). The release studies of CS-AG-CH bioscaffold demonstrate controlled release of CH with in the bioscaffold at different pH conditions. The bioscaffold demonstrated effective antibacterial activity against S. aureus and E. coli strains. Additionally, cytotoxicity assays indicated that the bioscaffold supports better cell viability and proliferation in fibroblast (NIH 3T3) cell lines. Consequently, this antimicrobial bioscaffold shows promise as a drug release system and biocompatible wound dressing suitable for tissue engineering applications.