AI Article Synopsis
- Lepidopteran pests are major threats to sunflowers, significantly damaging crops.
- Researchers developed a transgenic sunflower, MCPN-7, using the Cry1Ab-Vip3Af2 gene to enhance resistance against pests like the yellow peach moth and cotton bollworm.
- Laboratory tests showed 100% mortality of these pests on MCPN-7 within 72 hours, and field trials indicated no significant damage, highlighting its potential for commercial use.
Article Abstract
Lepidopteran pests frequently cause significant damage to Sunflowers (Helianthus annuus). In this study, the insect resistant fusion gene Cry1Ab-Vip3Af2 was transformed into sunflower by Agrobacterium-mediated transformation. A transgenic event, named MCPN-7, was selected and characterized for its high resistance to both yellow peach moth (Dichocrocis punctiferalis) and cotton bollworm (Helicoverpa armigera), two polyphagous pests feeding on various plants including sunflower. The neonates of both species feeding on MCPN-7 resulted to 100 % mortality within 72 h in laboratory bioassays. No significant damage caused by the two insects was observed in field trials of MCPN-7. ELISA analysis revealed that the fusion protein was predominantly expressed in leaves, seeds and heads. The flanking genomic sequence of the T-DNA of the event MCPN-7 was determined and confirmed by PCR analysis. In conclusion, the transgenic sunflowers obtained in this study is highly resistant to wide spectrum of Lepidopteran insect pests and could potentially be a candidate event for commercial development.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.136219 | DOI Listing |
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