Identification of Key Genes Controlling Sugar and Organic Acid Accumulation in Wampee Fruit () via Genome Assembly and Genome-wide Association Analysis.

Wampee () is an economically significant subtropical fruit tree widely cultivated in Southern China. To provide high-quality genomic resources for , we report a chromosome-level genome sequence for the "JinFeng" cultivar. The 297.1 Mb genome contained nine chromosomes with a scaffold N50 of 29.2 Mb and encoded 23,468 protein-coding genes. Selective sweep analysis between sweet and sour varieties and genome-wide association analysis identified 14 candidate genes putatively involved in sugar and acid accumulation. , encoding an ethylene response factor, and , encoding a Sugars Will Eventually be Exported Transporters (SWEET) family protein, were proposed as key regulators of the sweet and sour tastes of the wampee fruit. and overexpression in tomatoes increased the total sugar and acid content in fruits. promoter activation by ClERF061 was confirmed via transient expression. Our study provides valuable genomic resources for genetics and breeding.