AI Article Synopsis
- Capillary electrophoresis-mass spectrometry (CE-MS) is an advanced analytical technique beneficial for clinical research and diagnostics, enhancing the identification of compounds in clinical samples.
- The ability of CE to separate molecules and MS to accurately identify and quantify these compounds makes it effective for analyzing complex biological samples like human plasma.
- A detailed protocol for conducting untargeted metabolomics studies using CE-MS is provided, focusing on its application to human plasma from Long COVID patients, covering everything from sample preparation to data interpretation.
Article Abstract
Capillary electrophoresis coupled to mass spectrometry (CE-MS) has emerged as a powerful analytical technique with significant implications for clinical research and diagnostics. The integration of information from CE and MS strengthens confidence in the identification of compounds present in clinical samples. The ability of CE to separate molecules based on their electrophoretic mobility coupled to MS enables the accurate identification and quantification of analytes, even in complex biological matrices such as human plasma.Here, we present a detailed protocol for an untargeted metabolomics study using CE-MS and its application in a study on human plasma from patients suffering Long COVID syndrome. The protocol ranges from sample preparation to biological interpretation, detailing a workflow enabling the analysis of cationic and anionic compounds, metabolite identification, and data processing.
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| http://dx.doi.org/10.1007/978-1-0716-4116-3_23 | DOI Listing |
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