Analysis of the Mammalian Lipidome by DMS Shotgun Lipidomics.

Methods Mol Biol

Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

Published: October 2024

Shotgun Lipidomics is a robust methodology for the characterization of the lipidome of complex biological samples. This assay is among the most quantitative lipidomics methods and is capable of surveying a wide breadth of lipid subclasses, both neutral and polar. The shortfalls of the technique include limitations in lipid species characterization and computationally demanding data analysis requiring isotopic and isobaric overlap correction. Differential Mobility Spectrometry (DMS) has demonstrated its utility in enabling acyl tail characterization within a Shotgun Lipidomics experiment. Here, we present a workflow for DMS Shotgun Lipidomics that measures 1400 possible lipid species. It utilizes the Shotgun Lipidomics Assistant (SLA) application, an open-source application that supervises the data analysis for an expansive Shotgun Lipidomics experiment.

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http://dx.doi.org/10.1007/978-1-0716-4116-3_21DOI Listing

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