In this chapter, we describe a multi-purpose, reversed-phase liquid chromatography-high-resolution mass spectrometry (LC-HRMS) workflow for acquiring high-quality, non-targeted exposomics data utilizing data-dependent acquisition (DDA) combined with the use of toxicant inclusion lists for semi-targeted analysis. In addition, we describe expected retention times for >160 highly diverse xenobiotics in human plasma and serum samples. The method described is intended to serve as a generic LC-HRMS exposomics workflow for research and educational purposes. Moreover, it may be employed as a primer, allowing for further adaptations according to specialized research needs, e.g., by including reference and/or internal standards, by expanding to data-independent acquisition (DIA), or by modifying the list of compounds prioritized in fragmentation experiments (MS).
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-0716-4116-3_3 | DOI Listing |
J Expo Sci Environ Epidemiol
December 2024
Department of Diagnostics and Intervention, Oncology, Umeå University, Umeå, 901 87, Sweden.
Background: The chemical exposome includes exposure to numerous environmental and endogenous molecules, many of which have been linked to reproductive outcomes due to their endocrine-disrupting properties. As several breast cancer risk factors, including age and parity, are related to reproduction, it is imperative to investigate the interplay between such factors and the chemical exposome prior to conducting large scale exposome-based breast cancer studies.
Objective: This pilot study aimed to provide an overview of the chemical exposome in plasma samples from healthy women and identify associations between environmental exposures and three risk factors for breast cancer: age, parity, and age at menarche.
Anal Bioanal Chem
November 2024
Department of Analytical Chemistry, Institute of Chemistry for Energy and the Environment, Universidad de Córdoba, Anexo Marie Curie, Campus de Rabanales, Córdoba, 14071, Spain.
The characterization of the human chemical exposome through daily estimated intakes or biomonitoring has become paramount to understand the causal pathways leading to common diseases. The paradigm shift that has taken place in looking at health has moved research from the classical biomedical model based on "one exposure, one disease" to a more comprehensive approach based on multiple chemicals and low dose effects. For this purpose, untargeted and/or suspect analysis of chemicals based on liquid chromatography and high-resolution mass spectrometry (LC-HRMS) has been proposed as the most relevant strategy for sequencing the exposome.
View Article and Find Full Text PDFAm J Hum Biol
January 2025
Chaco Area Reproductive Ecology Program, Formosa, Argentina.
Objective: Exposure to environmental contaminants is globally universal. However, communities vary in the specific combination of contaminants to which they are exposed, potentially contributing to variation in human health and creating "locally situated biologies." We investigated how environmental exposures differ across environments by comparing exposure profiles between two contexts that differ markedly across political, economic, and sociocultural factors-Namqom, Formosa, Argentina, and New Haven, Connecticut, United States.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
October 2024
ABF Analytisch-Biologisches Forschungslabor GmbH, Semmelweisstraße 5, 82152 Planegg, Germany. Electronic address:
The global prevalence of electronic cigarettes, heated tobacco products, and other smokeless alternatives has grown significantly in the last ten years. These products have been suggested as combustion-free alternatives for conventional tobacco products like cigarettes, aiming to reduce the negative health impacts associated with smoking. However, the impact of those products on the health and safety of the general population are still unclear, as the absolute exposure from those products has not been thoroughly studied, yet.
View Article and Find Full Text PDFMethods Mol Biol
October 2024
Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Vienna, Austria.
In this chapter, we describe a multi-purpose, reversed-phase liquid chromatography-high-resolution mass spectrometry (LC-HRMS) workflow for acquiring high-quality, non-targeted exposomics data utilizing data-dependent acquisition (DDA) combined with the use of toxicant inclusion lists for semi-targeted analysis. In addition, we describe expected retention times for >160 highly diverse xenobiotics in human plasma and serum samples. The method described is intended to serve as a generic LC-HRMS exposomics workflow for research and educational purposes.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!