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The influence of fetal bovine serum concentration on stemness and neuronal differentiation markers in stem cells from human exfoliated deciduous teeth. | LitMetric

AI Article Synopsis

  • - Dental Stem Cells (DSCs) from discarded teeth, especially Stem cells from human exfoliated deciduous teeth (SHED), are valued for their potential in nerve-related regeneration due to their unique origin and differentiation capabilities.
  • - Key markers CD56 and CD271 are crucial for identifying specific stem cell subpopulations and enhancing their therapeutic applications in neurodevelopment.
  • - The study finds that lower concentrations of fetal bovine serum (FBS) boost the expression of CD56 and CD271 in SHED, optimizing their culture conditions for better use in regenerative medicine and tissue engineering.

Article Abstract

Dental Stem Cells (DSCs) from discarded teeth are a non-invasive and ethically favorable source with the potential for neurogenesis due to their ectodermal origin. Stem cells from human exfoliated deciduous teeth (SHED) are particularly promising due to their high differentiation potential and relative immaturity compared to other Mesenchymal Stromal Cells (MSCs). Markers like CD56 and CD271 are critical in identifying MSC subpopulations for therapeutic applications because of their roles in neurodevelopment and maintaining stemness. This study investigates how fetal bovine serum (FBS) concentrations affect the expression of CD56 and CD271 in SHED, influencing their stemness and neuronal differentiation potential. SHEDs were isolated from various donors, cultured, and characterized for MSC traits using markers such as CD14, CD19, CD29, CD34, CD45, CD73, CD90, CD105, CD56, and CD271. Culturing SHED in different FBS conditions (standard 15 %, reduced 1 % and 5 %, and FBS-free) showed that lower FBS concentrations increase CD271 and CD56 expression while maintaining the standard MSC immunophenotype. Importantly, the enhanced expression of these markers can be induced even after SHEDs have been expanded in standard FBS concentrations. These findings suggest that FBS concentration can optimize SHED culture conditions, enhancing their suitability for regenerative medicine and tissue engineering applications.

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Source
http://dx.doi.org/10.1016/j.tice.2024.102571DOI Listing

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