AI Article Synopsis
- Cancer-associated extracellular vesicles (EVs) provide vital tumor information for cancer diagnosis, and a new electrochemical biosensor has been developed to detect them effectively.
- The sensor uses an anti-CXCR4 antibody on a gold-plated electrode to capture EVs and employs Fe/Cu diatomic catalysts for sensitive measurement of electrical signals linked to EV concentration.
- The device shows a reliable linear response in detecting EVs across a concentration range, boasts a low detection limit, and offers advantages like affordability and ease of use for clinical applications.
Article Abstract
Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis as they contain abundant tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR)-based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst strategies for catalytic HPRR current amplification for specific identification and highly sensitive detection. The anti-CXCR4 antibody was immobilized on a Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bound to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from the HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 1 × 10 particles mL, with a detection limit of 117 particles mL, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.
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| http://dx.doi.org/10.1039/d4ay01573h | DOI Listing |
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