AI Article Synopsis

  • Bacterial keratitis is a serious eye infection that can lead to corneal inflammation and potentially blindness, but can be treated with topical fluoroquinolones and corticosteroids like Gatifloxacin and Dexamethasone.
  • A new bioanalytical method using tandem mass spectrometry (LC-MS/MS) has been developed to simultaneously measure Gatifloxacin and Dexamethasone, achieving separation in under 5 minutes with high accuracy.
  • The method was validated according to USFDA guidelines and applied successfully in a study analyzing these drugs in rabbit tear fluid after topical application, demonstrating its effectiveness for pharmacokinetic analysis.

Article Abstract

Bacterial keratitis (BK) is an infection that causes inflammation of the cornea and, if severe, can result in blindness. Topical fluoroquinolones combined with corticosteroids have been shown to be useful in the treatment of BK. A rapid, selective, and sensitive bioanalytical method for simultaneous quantification of Gatifloxacin (GAT) and Dexamethasone (DEX) has been developed and validated using tandem mass spectrometry (LC-MS/MS). Optimal separation was accomplished in under 5 min using an Agilent Zorbax C18 column (100 mm × 4.6 mm, 3.5 μm). The mobile phase was composed of a blend of 0.2% formic acid in triple distilled water and methanol with a flow rate of 0.65 mL/min in isocratic mode. GAT and DEX were detected in positive electrospray ionization multiple reaction monitoring mode (MRM), and the retention time was found to be at 1.64 and 2.93 min, respectively. The linearity of GAT and DEX was found to be in the range of 1.56-400 ng mL with good precision and accuracy. The method was validated according to USFDA regulatory guidelines. The validated method was effectively utilized for preclinical pharmacokinetic analysis of GAT and DEX in rabbit tear fluid following the topical application of a commercial formulation.

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Source
http://dx.doi.org/10.1002/jms.5088DOI Listing

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