controls determinate inflorescence trait in .

Unlabelled: Determinate inflorescence is indeed a pivotal agricultural characteristic in crops, notably impacting the architecture modification of (AACC, 2n = 38). Previous study identified a crucial gene that encodes the transcription factor (). Here by two alleles were cloned and sequenced from indeterminate 2982 and determinate 4769, respectively, we found that harbors two T/C and G/C non-synonymous mutations in exon 1, and contains sixty-six differences in a 1.9 Kb promoter sequence. Subsequently, was introduced into 571 line by genetic complementation and overexpression, transgenic plants 571 lines and 571lines were all restored to the indeterminate inflorescence. Interestingly, after was knocked out in 'Westar', transgenic plants Westar lines were mutated to determinate inflorescences. Additionally, a NIL-4769 line was constructed to evaluate the effect of on agronomic traits of , the results demonstrated that reduced the plant height and increased the branch number and branch thousand grain weight of Finally, we performed RT-qPCR, GUS staining and subcellular localization experiments to analyze the expression pattern of , the results showed that the expression of at shoot apex of NIL-4769 was higher than that of 4769, GUS activity was detected at apical of and was detected in cell membrane, nucleus and cytoplasm. Our findings provide a firm molecular foundation for the study of rapeseed's molecular mechanism of determinate inflorescence formation, as well as theoretical guidance for the application of determinate inflorescence in rapeseed breeding.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-024-01503-7.