AI Article Synopsis
- The study investigates the causes of different types of gallstones in populations living at high altitudes from a molecular perspective.
- Bile samples from 20 cholesterol gallstone and 20 pigment gallstone disease patients were analyzed using advanced proteomics and metabolomics techniques, revealing numerous differentially expressed proteins and metabolites.
- Key findings include the identification of potential biomarkers and significant pathways involved in gallstone formation, contributing to a better understanding of the disease mechanisms in these populations.
Article Abstract
Background: To explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.
Methods: We collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.
Results: We identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.
Conclusion: The findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11440720 | PMC |
| http://dx.doi.org/10.1186/s12876-024-03422-5 | DOI Listing |
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