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Optimization of FRET imaging in Arabidopsis Protoplasts.
Mol Cells
January 2025
Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea; Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea; Institute of Systems Biology, Pusan National University, Busan 46241, Republic Korea. Electronic address:
Recent advancements in fluorescence-based biosensor technologies have enabled more precise and accurate Förster Resonance Energy Transfer (FRET) imaging within Agrobacterium-mediated plant transformation systems. However, the application of FRET imaging in plant tissues remains hindered by significant challenges, particularly the time-intensive process of generating transgenic lines and the complications arising from tissue autofluorescence. In contrast, protoplast-based FRET imaging offers a rapid and efficient platform for functional screening and analysis, making it an essential tool for plant research.
View Article and Find Full Text PDFSmartphone-assisted hydrogel sensing platform based on double emission carbon dots for portable on-site tetracycline detection.
Mikrochim Acta
January 2025
Department of Chemistry and Material Engineering, Lyuliang University, Lyuliang, 033000, P. R. China.
Innovative double-emission carbon dots (DE-CDs) were synthesized via a one-step hydrothermal method using fennel and m-phenylenediamine (m-PD) as precursors. These DE-CDs exhibited dual emission wavelengths at 432 and 515 nm under different excitations, making them highly versatile for fluorescence-based applications. The fluorescence of the DE-CDs was efficiently quenched by tetracycline (TC) through the inner filter effect (IFE), allowing for the construction of a sensitive dual-response fluorescent sensor.
View Article and Find Full Text PDFRecent Advances in Fluorescent Based Chemical Probes for the Detection of Perchlorate Ions.
Crit Rev Anal Chem
January 2025
SIMETRI, Inc, Winter Park, Florida, USA.
This review highlights recent advancements and challenges in fluorescence-based chemical sensors for selective and sensitive detection of perchlorate, a persistent environmental pollutant and global concern due to its health and safety implications. Perchlorate is a highly persistent inorganic pollutant found in drinking water, soil, and air, with known endocrine-disruptive properties due to its interference with iodide uptake by the thyroid gland. Human exposure mainly occurs through contaminated water and food.
View Article and Find Full Text PDFNanoconfined Chlorine-Substituted Monomethine Cyanine Dye with a Propionamide Function Based on the Thiazole Orange Scaffold-Use of a Fluorogenic Probe for Cell Staining and Nucleic Acid Visualization.
Molecules
December 2024
Faculty of Chemistry and Pharmacy, Sofia University "St. Kliment Ohridski", 1 James Bourchier Blvd., 1164 Sofia, Bulgaria.
The development of fluorescence-based methods for bioassays and medical diagnostics requires the design and synthesis of specific markers to target biological microobjects. However, biomolecular recognition in real cellular systems is not always as selective as desired. A new concept for creating fluorescent biomolecular probes, utilizing a fluorogenic dye and biodegradable, biocompatible nanomaterials, is demonstrated.
View Article and Find Full Text PDFExploring Benzothieno[3,2-b]benzothiophene S-Oxides for Organic Electronics, Fluorescence-Based Applications, and Asymmetric Synthesis.
Chemistry
December 2024
Division of Organic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363, Lodz, Poland.
A series of benzothieno[3,2-b]benzothiophene S-oxides has been efficiently synthesized via one- or two-step selective oxidation of benzothieno[3,2-b]benzothiophene (BTBT). These BTBT S-oxides form highly ordered structures that display optical anisotropy, are thermally stable up to temperatures above 210 °C, and do not show phase transitions. They exhibit long excited state lifetimes (0.
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