Antioxidant Features of Humic Products by ABTS Assay.

Methods Mol Biol

Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.

Published: September 2024

AI Article Synopsis

  • Oxidative stress contributes to immunosenescence by disturbing cellular balance and weakening immune response.
  • Humic substances can scavenge reactive oxygen species, reduce ROS generation, and enhance the activity of antioxidant enzymes, promoting better cellular health.
  • This study utilizes the ABTS spectrophotometric assay to assess the antioxidant potential of humic products, highlighting their potential as natural remedies for oxidative stress-related immune aging.

Article Abstract

Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune function. Humic substances exhibit scavenging activity against reactive oxygen species (ROS), inhibit ROS generation via metal chelation, and modulate endogenous antioxidant enzyme activity. Additionally, humic substances display anti-inflammatory effects, further supporting cellular redox balance. Given their antioxidant activity, humic substances hold promise as natural compounds for mitigating oxidative stress-associated immunosenescence. Here we describe the evaluation of antioxidant capacities of humic products by ABTS spectrophotometric assay.

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http://dx.doi.org/10.1007/978-1-0716-4128-6_19DOI Listing

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