AI Article Synopsis
- Ischemia-reperfusion (I/R) injury can cause severe testicular damage and infertility, prompting research on fasudil as a potential protective treatment.
- The study involved 30 male Long-Evans rats divided into groups to compare the effects of fasudil treatment before and after I/R injury against control and untreated injured groups.
- Results showed that fasudil significantly reduces indicators of tissue damage and inflammation, especially when given before detorsion, suggesting it offers protection against I/R injury in testicular tissue.
Article Abstract
Background: Ischemia-reperfusion (I/R) injury to the testis can lead to organ damage, infertility, and subfertility. The goal of this study was to investigate the effects of fasudil on this devastating condition.
Methods: Thirty male Long-Evans rats were divided into five groups: a control group (no torsion), rats administered fasudil (30 mg/kg, no torsion), rats subject to ischemia with no treatment (I) (I/R injury), injured rats that received treatment 1 (T1) (I/R with 30 mg/kg fasudil before detorsion), and injured rats that received treatment 2 (T2) (I/R with 30 mg/kg fasudil after detorsion). Serum levels of TNF-ɑ and IL-6, along with tissue levels of glutathione (GSH), malondialdehyde (MDA), caspase-3, and Johnsen Tubular Biopsy Score (JTBS), were measured.
Results: Group I exhibited significantly higher levels of MDA and caspase-3 than all other groups except T2 (p ˂ 0.05). Although the difference was not statistically significant, Group T2 exhibited lower MDA and caspase-3 levels than Group I (p ˃ 0.05). Additionally, Group I displayed significantly higher TNF-ɑ and IL-6 levels, and lower GSH and JTBS values, than the other groups (p ˂ 0.05).
Conclusion: Our findings indicate that fasudil protects the testis from I/R injury, particularly when administered early.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439356 | PMC |
| http://dx.doi.org/10.2147/DDDT.S480774 | DOI Listing |
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