AI Article Synopsis
- No approved human vaccine exists for Venezuelan equine encephalitis (VEE), which is caused by the VEE virus (VEEV).
- The study employed high-fidelity PCR to create synthetic fragments of the VEEV genome to aid in developing a live virus vaccine.
- Results showed that very small amounts of these synthetic fragments could successfully initiate the replication of the vaccine virus in cell cultures, suggesting a potential new method for vaccine development against VEE and other viruses.
Article Abstract
Background/objectives: There is no approved human vaccine for Venezuelan equine encephalitis (VEE), a life-threatening disease caused by the VEE virus (VEEV). In previous studies, plasmid DNA encoding the full-length RNA genome of the VEE V4020 vaccine was used for the preparation of experimental live virus VEE vaccines in the plasmid-transfected cell culture.
Methods: Here, we used the high-fidelity polymerase chain reaction (PCR) to prepare synthetic, transcriptionally active PCR (TAP) fragments encoding the V4020 genome.
Results: TAP fragment initiated the replication of the V4020 live virus vaccine in TAP fragment-transfected cells. A transfection of less than 1 ug of TAP fragment resulted in the replication of the V4020 vaccine virus in CHO cells.
Conclusion: We conclude that not only plasmid DNA but also synthetic PCR-generated DNA fragments can be used for the manufacturing of live vaccines for VEEV and, potentially, other viruses.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434715 | PMC |
| http://dx.doi.org/10.3390/pharmaceutics16091217 | DOI Listing |
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