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Rapid and Accurate Ecotoxicological Assessment of Heavy Metals Using Cells. | LitMetric

AI Article Synopsis

  • Heavy metals negatively impact aquatic organisms, necessitating quick and precise ecotoxicological assessments.
  • Traditional fish-derived cell methods take at least 96 hours for toxicity testing, which can be slow for large assessments.
  • This study optimized testing conditions by using 1% fetal bovine serum and reducing treatment time to 6 hours, leading to faster and more accurate ecotoxicity results for heavy metals.

Article Abstract

Heavy metals have serious negative effects on various aquatic organisms, and therefore rapid and accurate ecotoxicological assessments of heavy metals are necessary. Fish-derived cells sensitive to heavy metals have been used as valuable tools for ecotoxicological assessments. However, this method requires a minimum toxicity treatment time of 96 h, which limits its use when rapid ecotoxicological assessments are required or ecotoxicological assessments of a large number of toxicants are performed. In this study, these limitations were overcome by adjusting parameters including the concentration of fetal bovine serum (FBS) in the medium and the treatment time of the toxicant. Specifically, we found that the maximum time for fish cells to remain unstarved was 6 h when using a medium containing 1% FBS. We applied both parameters to the ecotoxicological assessment (using a medium containing 1% FBS for the toxicity assessment and treating the toxicant for only 6 h). Surprisingly, these adjusted parameters allowed us to obtain faster and more accurate data than the traditional assessment. This improvement was due to the new assessment conditions that minimized the possibility that the growth-inducing effects of nutrients present in excess in the medium could interfere with the cellular response to the toxicant. The accuracy of this assessment was not limited to measuring the toxicity of heavy metals. In conclusion, we have established an ecotoxicity assessment that can generate rapid and accurate data on heavy metals. This new platform will become the cornerstone of rapid and accurate ecotoxicity assessments of heavy metals.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11432982PMC
http://dx.doi.org/10.3390/life14091119DOI Listing

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