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Study of the Synchronization and Transmission of Intracellular Signaling Oscillations in Cells Using Bispectral Analysis. | LitMetric

AI Article Synopsis

  • The study focuses on oscillation synchronization analysis in biological systems, aiming to understand how living organisms respond to environmental changes and its applications in medicine and agriculture.
  • Researchers are investigating the bispectral analysis method to better evaluate synchronized oscillations in cell biology, specifically in mouse microvessel endothelial cells responding to stress conditions like increased temperature and hyperglycemia.
  • Findings reveal that temperature elevation increases synchronization of calcium oscillations but reduces nitric oxide synchronization, with different impacts based on the environmental stressors, while the paper details the bispectral analysis methodology and compares it with other analytical techniques.

Article Abstract

The oscillation synchronization analysis in biological systems will expand our knowledge about the response of living systems to changes in environmental conditions. This knowledge can be used in medicine (diagnosis, therapy, monitoring) and agriculture (increasing productivity, resistance to adverse effects). Currently, the search is underway for an informative, accurate and sensitive method for analyzing the synchronization of oscillatory processes in cell biology. It is especially pronounced in analyzing the concentration oscillations of intracellular signaling molecules in electrically nonexcitable cells. The bispectral analysis method could be applied to assess the characteristics of synchronized oscillations of intracellular mediators. We chose endothelial cells from mouse microvessels as model cells. Concentrations of well-studied calcium and nitric oxide (NO) were selected for study in control conditions and well-described stress: heating to 40 °C and hyperglycemia. The bispectral analysis allows us to accurately evaluate the proportion of synchronized cells, their synchronization degree, and the amplitude and frequency of synchronized calcium and NO oscillations. Heating to 40 °C increased cell synchronization for calcium but decreased for NO oscillations. Hyperglycemia abolished this effect. Heating to 40 °C changed the frequencies and increased the amplitudes of synchronized oscillations of calcium concentration and the NO synthesis rate. The first part of this paper describes the principles of the bispectral analysis method and equations and modifications of the method we propose. In the second part of this paper, specific examples of the application of bispectral analysis to assess the synchronization of living cells in vitro are presented. The discussion compares the capabilities of bispectral analysis with other analytical methods in this field.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11428995PMC
http://dx.doi.org/10.3390/biology13090685DOI Listing

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