Antibacterial and Antibiofilm Potential of Chlorophyllin Against In Vitro and In Silico.

Background: is a leading causative agent of dental caries and exerts pathogenicity by forming biofilms. Dental caries continues to be a significant public health issue worldwide, affecting an estimated 2.5 billion people, showing a 14.6% increase over the past decade. Herein, the antibacterial potential of Chlorophyllin extracted from was evaluated against biofilm-forming via in vitro and in silico studies.

Methodology: The antimicrobial activity of chlorophyllin extract against isolates was tested using the agar well diffusion method. Chlorophyllin extract was also tested against biofilm-forming isolates of . Chlorophyllin was docked with the antigen I/II (AgI/II) protein of to evaluate its antimicrobial mechanism. The chemical structure and canonical SMILES format of Chlorophyllin were obtained from PubChem. Additionally, adsorption, distribution, metabolism, excretion, and toxicity (ADMET) analyses of Chlorophyllin were performed using ADMETlab 2.0 to assess its pharmacokinetic properties.

Results: An agar well diffusion assay revealed that all isolates were susceptible to Chlorophyllin extract and showed a variety of inhibition zones ranging from 32 to 41 mm. Chlorophyllin reduces the biofilm strength of four isolates from strong to moderate and six from strong to weak. The antibiofilm potential of Chlorophyllin was measured by a reduction in the number of functional groups observed in the Fourier Transform Infrared Spectrometer (FTIR) spectra of the extracellular polymeric substance (EPS) samples. Chlorophyllin showed binding with AgI/II proteins of , which are involved in adherence to the tooth surface and initiating biofilm formation. The ADMET analysis revealed that the safety of Chlorophyllin exhibited favorable pharmacokinetic properties.

Conclusions: Chlorophyllin stands out as a promising antibacterial and antibiofilm agent against biofilm-forming , and its safety profile highlights its potential suitability for further investigation as a therapeutic agent.