AI Article Synopsis
- Adenoviruses are important tools in gene therapy and vaccine development, but their production poses challenges, particularly the risk of generating replication-competent adenoviruses (RCAs) in common cell lines like HEK293A.
- To address this issue, the study focused on creating a new cell line, L132, derived from human primary cells and enhanced by retroviral transduction.
- The newly developed GT541 cell line shows promise as an effective alternative to HEK293A for producing RCA-free adenoviruses.
Article Abstract
Adenoviruses are commonly utilized as viral vectors for gene therapy, genetic vaccines, and recombinant protein expression. To generate replication-defective adenoviruses, E1-complementing cell lines such as HEK293A are utilized; however, limitations remain. Repeated passage of E1-deleted virus in HEK293A cells increases the occurrence of replication-competent adenoviruses (RCAs). In the present study, we developed a novel cell line originating from human primary cells. L132 cells were transduced two times with E1-encoded retrovirus and three times with E1A-encoded retrovirus. Finally, we selected the most productive L132 cell line for generation of RCA-free adenovirus, GT541. GT541 can serve as an alternative cell line to HEK293A and other adenovirus-producing cells.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11429178 | PMC |
| http://dx.doi.org/10.1186/s12896-024-00894-x | DOI Listing |
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