AI Article Synopsis
- - The study explores the potential of extracellular vesicles from human amniotic epithelial cells (hAEC-EVs) as a treatment for Dry Eye Disease (DED).
- - Researchers isolated and characterized hAEC-EVs, finding proteins that promote cell proliferation and reduce inflammation. They tested the efficacy of these vesicles on both human corneal epithelial cells and in a mouse model of DED.
- - Results showed that hAEC-EVs significantly improved corneal health metrics in mice and increased healing and reduced inflammation in ocular cells, suggesting a promising new approach for treating DED.
Article Abstract
Purpose: This study aimed to investigate the therapeutic potential of extracellular vesicles (EVs) derived from human amniotic epithelial cells (hAEC-EVs) for Dry Eye Disease (DED) treatment.
Methods: Highly purified EVs were isolated from the culture supernatants of hAECs, which obtained from term placenta and characterized. Proteomic contents were analyzed for assessing its biological function related to the therapeutic potentials for DED. Subsequently, we examined the therapeutic efficacy of hAEC-EVs on human corneal epithelial cells exposed to hyperosmotic stress and in an experimental DED mouse model induced by desiccation stress.
Results: Proteomic analysis of hAEC-EVs revealed proteins linked to cell proliferation and anti-inflammatory responses. We demonstrated efficient uptake of hAEC-EVs by ocular surface cells. Under DED conditions, EV treatment increased corneal epithelial cell proliferation and migration, and concurrently reducing inflammatory cytokines. In the DED mouse model, hAEC-EVs showed significant improvements in corneal staining score, tear secretion, corneal irregularity, and conjunctival goblet cell density. Additionally, hAEC-EVs exhibited a mitigating effect on ocular surface inflammation induced by desiccation.
Conclusions: These findings suggest that hAEC-EVs hold potential as a cell-free therapy for corneal epithelial defects and ocular surface diseases, presenting a promising treatment option for DED.
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| http://dx.doi.org/10.1016/j.jtos.2024.09.006 | DOI Listing |
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