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Reproductive biotechnologies are widely consolidated as a methodology in cattle breeding and have an important impact on the genetic improvement of cattle herds. Semen is an important source of dissemination of pathogenic microorganisms during reproductive procedures. To ensure the sanitary quality of the semen, it is essential to consider the presence of various microorganisms including viruses. One of the main viral agents of reproductive interest is Bovine Alphaherpesvirus 1 (BoHV-1), the etiological agent responsible for bovine rhinotracheitis and vulvovaginitis and frequently associated with reproductive efficiency of matrices and bulls. In artificial insemination centers, semen treatment is generally based only on the use of antibiotics, ignoring the possibility of inactivating other non-bacterial infectious agents. In this context, photodisinfection emerges as a promising alternative to inactivate a wide range of microorganisms, offering a complementary or substitution approach to those conventional semen treatment methods. In this work, we evaluated the use of four halogenated sulfonated porphyrins as potential photosensitizers (PSs) for photodynamic inactivation of Bovine Alphaherpesvirus I (BoHV-1) for bovine semen disinfection. The PSs were synthesized and photophysical parameters, such as UV-Vis absorption spectra and singlet oxygen quantum yield (Φ) were presented. Photoinactivation of BoHV-1 was first shown in cell culture and then confirmed in artificially infected bovine semen and then the phototoxicity of PSs against spermatozoa was evaluated. All PSs were effective in BoHV-1 inactivation; however, the photosensitizer containing two chlorine atoms, showed to be more efficient due to the shorter time required for complete viral inactivation. The slight alterations in sperm kinetics were observed, but remained within those acceptable by regulatory agencies for animal reproduction. Although the methodology used in this work only included bovine semen, we emphasize that the proposed photodisinfection methodology can be adapted and applied to a wide range of biological materials and microorganisms of animal or human interest.
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http://dx.doi.org/10.1016/j.jphotobiol.2024.113036 | DOI Listing |
Theriogenology
December 2024
Universidade Federal de Mato Grosso (UFMT) campus Cuiabá, Avenida Fernando Corrêa da Costa, 2367, Boa Esperança, Cuiabá, 78060-900, Brazil; Programa de Pós-Graduação Stricto Sensu em Biociência Animal, Universidade de Cuiabá (UNIC), Avenida Manoel José de Arruda, 3100, Jardim Europa, Cuiabá, 78065-900, Brazil. Electronic address:
This study aimed to evaluate the impact of cholesterol supplementation at various concentrations in cryopreserved Pantaneiro bovine semen on in vitro embryo production (IVEP). Grade I and II cumulus-oocyte complexes (COCs) were collected from ovaries retrieved from a commercial slaughterhouse and matured in vitro for 24 h. The matured COCs were divided into four groups based on the concentration of cholesterol -loaded cyclodextrin (CLC) during semen cryopreservation from a Pantaneiro breed bull: Control (C) - 0 mg/mL CLC, T1 - 0.
View Article and Find Full Text PDFReprod Domest Anim
December 2024
Animal Reproduction, Gynaecology and Obstetrics, Artificial Breeding Research Centre (ABRC), ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Bull fertility is a multi-factorial trait and is affected by many factors, such as nutrition, genetics, and epigenetics. Superior quality male germplasm with high genetic merit helps to improve the livestock production trait. To achieve the target of livestock production, the availability of superior male germplasm is a great concern.
View Article and Find Full Text PDFJ Anim Sci
December 2024
North Florida Research and Education Center, University of Florida, Marianna, Florida.
Changes in maternal nutrition during the periconceptional period can influence postnatal growth in cattle. This study aimed to identify the impact of supplementing beef cows with rumen-protected methionine (RP-Met) during the periconceptional period on their female progeny. In exp 1, plasma methionine (Met) levels were analyzed in samples from 10 Angus crossbred, non-lactating beef cows.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Molecular Genetics Group, Institute of Animal Breeding and Husbandry, Christian-Albrechts-University Kiel, 24118 Kiel, Germany.
Sperm mitochondria are vital organelles for energy production and pre- and post-fertilization sperm functions. The potential influence of the age of the bull and season on the sperm-borne mitochondrial copy number and the transcription activity has not yet been investigated. Therefore, the expression patterns of all protein-coding mitochondrial genes were identified throughout the year along with mitochondrial copy numbers in young and old bulls' spermatozoa.
View Article and Find Full Text PDFJ Reprod Dev
December 2024
Global Agromedicine Research Center (GAMRC), Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Artificial insemination (AI) in cattle involves introducing frozen-thawed sperm, a minimal amount of seminal plasma, and a significant volume of semen extender (SE) into the uterus. Previous studies have demonstrated that sperm interacts with bovine endometrial epithelia via TLR 2/1, triggering a weak inflammatory response to clear the endometrium. This study investigated the impact of the major component of the insemination dose, egg yolk-based SE, on the uterine immune response in vitro.
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