Innovative approach of nanopore-based metagenomic sequencing for rapid identification of bacterial pathogens in joint fluid: A pilot study.

Diagn Microbiol Infect Dis

Institute of Emerging Infectious Diseases, Korea University, Seoul, Republic of Korea; Division of Infectious Diseases, Department of Internal Medicine, Korea University Anam Hospital, Korea University College of Medicine, Seoul, Republic of Korea. Electronic address:

Published: December 2024

Analysis of 11 clinical samples of joint fluid in this pilot study demonstrated proof-of-concept for nanopore-based metagenomic sequencing to serve as a complementary real-time diagnostic technique for septic arthritis, with a sensitivity of 75.0 % and specificity of 57.1 %, compared to the gold standard method of bacterial culture.

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http://dx.doi.org/10.1016/j.diagmicrobio.2024.116545DOI Listing

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