AI Article Synopsis
- Coumaric acid is important for various industries but traditional production methods are not very efficient.
- This study explores improving coumaric acid production by using a genetically engineered strain of bacteria (KT2440) that expresses a specific enzyme (TAL) to convert L-tyrosine into coumaric acid.
- The researchers achieved successful integration of the TAL gene and optimized production through a pH shift during fermentation, resulting in a significant yield of coumaric acid (1381 mg/L), showcasing the potential for sustainable production methods.
Article Abstract
Coumaric acid (-CA) is a valuable compound with applications in food additives, cosmetics, and pharmaceuticals. However, traditional production methods are often inefficient and unsustainable. This study focuses on enhancing -CA production efficiency through the heterologous expression of tyrosine ammonia-lyase (TAL) from in KT2440. TAL catalyzes the conversion of L-tyrosine into -CA and ammonia. We engineered KT2440 to express TAL in a fed-batch fermentation system. Our results demonstrate the following: (i) successful integration of the TAL gene into KT2440 and (ii) efficient bioconversion of L-tyrosine into -CA (1381 mg/L) by implementing a pH shift from 7.0 to 8.5 during fed-batch fermentation. This approach highlights the viability of KT2440 as a host for TAL expression and the successful coupling of fermentation with the pH-shift-mediated bioconversion of L-tyrosine. Our findings underscore the potential of genetically modified for sustainable -CA production and encourage further research to optimize bioconversion steps and fermentation conditions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11430055 | PMC |
| http://dx.doi.org/10.3390/cimb46090603 | DOI Listing |
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