AI Article Synopsis
- - Biocatalysis is becoming a key part of modern organic chemistry, offering a more efficient and eco-friendly way to carry out chemical reactions.
- - Advances in molecular biology and protein engineering allow for the incorporation of noncanonical amino acids, expanding beyond the traditional 20 and leading to new possibilities in enzyme applications.
- - The review explores the effects of these noncanonical amino acids on enzymatic reactions, including the development of new active sites and innovative reactions, while also addressing the current challenges and future opportunities in this field.
Article Abstract
Biocatalysis has become an important component of modern organic chemistry, presenting an efficient and environmentally friendly approach to synthetic transformations. Advances in molecular biology, computational modeling, and protein engineering have unlocked the full potential of enzymes in various industrial applications. However, the inherent limitations of the natural building blocks have sparked a revolutionary shift. genetic incorporation of noncanonical amino acids exceeds the conventional 20 amino acids, opening new avenues for innovation. This review provides a comprehensive overview of applications of noncanonical amino acids in biocatalysis. We aim to examine the field from multiple perspectives, ranging from their impact on enzymatic reactions to the creation of novel active sites, and subsequent catalysis of new-to-nature reactions. Finally, we discuss the challenges, limitations, and promising opportunities within this dynamic research domain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11467907 | PMC |
| http://dx.doi.org/10.1021/acs.chemrev.4c00136 | DOI Listing |
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