Antibiotic-induced dysbiosis in the SCIME™ recapitulates microbial community diversity and metabolites modulation of disease.

Establishing The Context: Intestinal dysbiosis is a significant concern among dog owners, and the gut health of pets is an emerging research field. In this context, the Simulator of the Canine Intestinal Microbial Ecosystem (SCIME™) was recently developed and validated with data.

Stating The Purpose/introducing The Study: The current study presents a further application of this model by using amoxicillin and clavulanic acid to induce dysbiosis, aiming to provoke changes in microbial community and metabolite production, which are well-known markers of the disease .

Describing Methodology: Following the induction of dysbiosis, prebiotic supplementation was tested to investigate the potential for microbiota recovery under different dietary conditions.

Presenting The Results: The results showed that antibiotic stimulation in the SCIME™ model can produce significant changes in microbial communities and metabolic activity, including a decrease in microbial richness, a reduction in propionic acid production, and alterations in microbial composition. Additionally, changes in ammonium and butyric acid levels induced by the tested diets were observed.

Discussing The Findings: This alteration in microbial community and metabolites production mimicks canine dysbiosis patterns. A novel dynamic model simulating canine antibiotic-induced dysbiosis, capable of reproducing microbial and metabolic changes observed , has been developed and is suitable for testing the effects of nutritional changes.