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Rational design of bioengineered recombinant collagen-like protein enhances GelMA hydrogel for diabetic wound healing. | LitMetric

Rational design of bioengineered recombinant collagen-like protein enhances GelMA hydrogel for diabetic wound healing.

Int J Biol Macromol

Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, School of Life Sciences, Inner Mongolia University, Hohhot, People's Republic of China. Electronic address:

Published: September 2024

AI Article Synopsis

  • Gelatin methacryloyl (GelMA) is promising for tissue engineering but lacks functional groups, limiting its clinical use.* -
  • Researchers created a recombinant collagen-like protein (RC) with biofunctional domains to enhance GelMA, which increased cell viability and migration in studies.* -
  • In vivo experiments showed that RC-modified GelMA improved wound healing in diabetic models by boosting key proteins, indicating its potential for broader applications in biomaterials.*

Article Abstract

Gelatin methacryloyl (GelMA) holds significant potential in tissue engineering; however, its clinical applications are often constrained by its lack of functional groups. To overcome this limitation, recombinant proteins with multiple biofunctional domains present a promising strategy for GelMA functionalization, enhancing its biological properties. In this study, we developed a rationally designed recombinant collagen-like protein (RC) engineered with multiple biofunctional domains, which demonstrated the ability to upregulate collagen 1α (COL-1α) expression in NIH-3 T3 cells. By utilizing EDC/NHS chemistry, the purified RC was conjugated to GelMA, resulting in GelMA-RC hydrogels that significantly improved cell viability and migration compared to unmodified GelMA. Subsequent in vivo studies showed that RC-modified GelMA exhibited superior wound healing efficacy, largely attributed to enhanced expression of cytokeratin-14 (CK-14) and COL-1α. These findings underscore the potential of RC-functionalized GelMA in promoting diabetic wound repair and suggest broader applicability for functionalizing other biomaterials.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2024.136012DOI Listing

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