Lignin: The green powerhouse for enzyme immobilization in biocatalysis and biosensing.

Enzymes play an important role in diverse industries and are critical components of many industrial products, yet, their application is limited due to their sensitivity to environmental conditions, recovery challenges, and susceptibility to inhibition. Immobilizing enzymes onto a suitable support matrix imparts higher resistance and improves operational flexibility, recyclability, and reusability. Lignin, a renewable and abundant biopolymer derived from the paper and pulp industry, has emerged as one of the prominent materials to be incorporated in support matrices. The distinctive characteristics of lignin include high mechanical strength, ease of separation, chemical stability, robust matrix for securing enzyme binding, biocompatibility, and ease of surface functionalization, making it a promising alternative to traditional synthetic materials. Research studies suggest the effectiveness of various lignin-based materials for immobilizing enzymes and significantly improving their stability, reusability, and catalytic activity. This article critically examines the unique properties of lignin and highlights significant contributions made in the development of enzyme immobilization for biocatalysis and biosensing applications. Additionally, the roles of hybrid materials, multienzyme immobilization, and innovative strategies like interfacial activation and enzyme shielding are discussed for overcoming the current challenges and developing sustainable, efficient, and robust biocatalytic and biosensing processes for industrial applications.