Pathways, challenges, and strategies for enhancing anaerobic production of short-chain and medium-chain carboxylic acids from algal slurry derived from wastewater.

Algal slurry (AS) generated from microalgae-based wastewater treatment processes holds significant potential for carboxylic acids production through anaerobic digestion (AD), which have emerged as promising products due to their high energy density, great economic value, and versatile applications. A comprehensive analysis of the pathways and optimization strategies for producing short-chain (SCCAs) and medium-chain (MCCAs) carboxylic acids using AS substrates is presented in this review. It begins by introducing and comparing two types of microalgae-based wastewater treatment processes: the microalgae process and the microalgal-bacterial consortia process. Afterwards, the review systematically examines the metabolic pathways involved in SCCAs and MCCAs production using AS substrates. Moreover, pretreatment strategies for enhancing the release of organic matter are critically discussed. Ultimately, specific emphasis is placed on addressing technical challenges and discussing future perspectives. This review provides a deeper understanding of the mechanisms and strategies involved in carboxylic acids production from wastewater-generated AS.