AI Article Synopsis
- - The study aimed to identify genes that are differently expressed in temporal artery biopsies (TABs) from patients with giant cell arteritis (GCA) showing varied inflammation patterns, specifically transmural inflammation (TMI) and inflammation limited to adventitia (ILA), compared to non-GCA controls.
- - An analysis of 770 immune-related genes revealed that TABs with TMI had significantly more gene expression changes (256 upregulated and 31 downregulated) versus normal TABs, while TABs with ILA mostly resembled normal samples with fewer significant changes.
- - The findings highlighted that TMI TABs exhibit a unique gene expression profile that contributes to understanding GCA's underlying mechanisms, as opposed to TABs with ILA
Article Abstract
Objective: To identify differentially expressed genes in temporal artery biopsies (TABs) from patients with giant cell arteritis (GCA) with different histological patterns of inflammation: transmural inflammation (TMI) and inflammation limited to adventitia (ILA), compared with normal TABs from patients without GCA.
Methods: Expression of 770 immune-related genes was profiled with the NanoString nCounter PanCancer Immune Profiling Panel on formalin-fixed paraffin-embedded TABs from 42 GCA patients with TMI, 7 GCA patients with ILA and 7 non-GCA controls.
Results: Unsupervised clustering of the samples revealed two distinct groups: normal TABs and TABs with ILA in one group, 41/42 TABs with TMI in the other one. TABs with TMI showed 31 downregulated and 256 upregulated genes compared with normal TABs; they displayed 26 downregulated and 187 upregulated genes compared with TABs with ILA (>2.0 fold changes and adjusted p values <0.05). Gene expression in TABs with ILA resembled normal TABs although 38 genes exhibited >2.0 fold changes, but these changes lost statistical significance after Benjamini-Yekutieli correction. Genes encoding TNF superfamily members, immune checkpoints, chemokine and chemokine receptors, toll-like receptors, complement molecules, Fc receptors for IgG antibodies, signalling lymphocytic activation molecules, JAK3, STAT1 and STAT4 resulted upregulated in TMI.
Conclusions: TABs with TMI had a distinct transcriptome compared with normal TABs and TABs with ILA. The few genes potentially deregulated in ILA were also deregulated in TMI. Gene profiling allowed to deepen the knowledge of GCA pathogenesis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423731 | PMC |
| http://dx.doi.org/10.1136/rmdopen-2024-004600 | DOI Listing |
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Article Synopsis
- - The study aimed to identify genes that are differently expressed in temporal artery biopsies (TABs) from patients with giant cell arteritis (GCA) showing varied inflammation patterns, specifically transmural inflammation (TMI) and inflammation limited to adventitia (ILA), compared to non-GCA controls.
- - An analysis of 770 immune-related genes revealed that TABs with TMI had significantly more gene expression changes (256 upregulated and 31 downregulated) versus normal TABs, while TABs with ILA mostly resembled normal samples with fewer significant changes.
- - The findings highlighted that TMI TABs exhibit a unique gene expression profile that contributes to understanding GCA's underlying mechanisms, as opposed to TABs with ILA
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