Microplastics abundance and potential ecological risk assessment in sediment, water and fish of Deepor Beel-a Ramsar Wetland of the Brahmaputra plain, India.

Microplastics (MPs) are increasingly recognized as environmental contaminants with complex impacts on fish and other aquatic organisms. This study determined the microplastics abundance and the induced-ecological risks of microplastics in water, sediment, and commonly harvested fishes of a Ramsar site, Deepor Beel of Assam, India. Six samples of water and sediment were collected with nine individuals of two commonly harvested fish species Puntius sophore (Pool Barb) and Gudusia chapra (Indian River Shad). The abundance of microplastics in water and sediments were analyzed through organic matter digestion using hydrogen peroxide (HO, 30%) and sodium chloride (NaCl) for density separation. Potassium hydroxide (KOH, 10%) was used for digestion of fish gut. The microplastics were identified visually and chemically characterized through micro-Raman spectroscopy. Total 467 microplastic particles in water and sediment, and 62 particles in fish were identified. An average concentration of 0.55 ± 0.06 particles/L in water, 4.03 ± 0.41 particles/100 g in sediment samples, 3.83 ± 2.26 particles/individual in Puntius sophore, and 6.5 ± 3.40 particles/individual in Gudusia chapra were detected. Fibers accounted to the major shape of microplastic in water (54%) and sediment (50%), whereas fragments (65%) were the major shapes detected in both fishes. The color composition includes blue, black, red, green, brown, yellow, and transparent. Fiber particles size ranged between 150 and 1782 µm, fragments within 85-325 µm, and sphere within 85-220 µm. Chemical characterization of microplastics revealed polymer types including polypropylene (PP = 27%), polyvinyl chloride (PVC = 25%), acrylonitrile-butadiene-styrene (ABS = 18%), polycarbonate (PC = 13%), polyethylene (12%), and polystyrene (PS = 5%). PHI levels were at hazard level III and V for water and sediment samples and at level IV for both fish species. The PLI at hazard level I indicated low pollution levels, whereas the PERI were within danger and extreme danger levels. This study is the first report in abundances of microplastics and the ecological risk assessment of microplastics in surface waters, sediments and fishes of Deepor Beel wetland.