AI Article Synopsis
- - Respiratory fungal infections pose a serious health risk and existing animal models don't accurately mimic human disease, prompting the need for better research models.
- - This study used primary human airway epithelial cells (hAECs) to examine responses to two important fungal pathogens through single-cell RNA sequencing.
- - Findings showed that while both fungi caused cellular stress and inflammation, they impacted different cell types and pathways, highlighting unique stress responses that could lead to potential treatment targets.
Article Abstract
Respiratory fungal infections pose a significant threat to human health. Animal models do not fully recapitulate human disease, necessitating advanced models to study human-fungal pathogen interactions. In this study, we utilized primary human airway epithelial cells (hAECs) to recapitulate the lung environment and investigate cellular responses to two diverse, clinically significant fungal pathogens, and . To understand the mechanisms of early pathogenesis for both fungi, we performed single-cell RNA sequencing of infected hAECs. Analysis revealed that both fungi induced cellular stress and cytokine production. However, the cell subtypes affected and specific pathways differed between fungi, with and triggering protein-folding-related stress in ciliated cells and hypoxia responses in secretory cells, respectively. This study represents one of the first reports of single-cell transcriptional analysis of hAECs infected with either or , providing a vital dataset to dissect the mechanism of disease and potentially identify targetable pathways.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418999 | PMC |
| http://dx.doi.org/10.1101/2024.09.09.612147 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anesthetic management for upper arm amputation in a patient with acute rapid atrial fibrillation and a large thyroid goiter: A case report.
Medicine (Baltimore)
January 2025
Department of Anesthesiology, Yanbian University Hospital, Yanji, Jilin, P.R. China.
Rationale: Patients with atrial fibrillation and a large goiter have high perioperative risks and often cannot tolerate general anesthesia, making it necessary for us to explore new safe and effective anesthesia methods.
Patient Concerns: The patient presented with atrial fibrillation accompanied by rapid ventricular rate, a thrombus attached to the left atrial appendage, and a massive thyroid goiter compressing the airway.
Diagnosis: After the left humerus fracture surgery, the patient's internal fixation loosened and fractured, accompanied by infection, formation of sinus tracts, and suppuration.
Epithelial-to-mesenchymal transition and live cell extrusion contribute to measles virus release from human airway epithelia.
J Virol
January 2025
Microbiology and Immunology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
Measles virus (MeV) is a highly contagious respiratory virus transmitted via aerosols. To understand how MeV exits the airways of an infected host, we use unpassaged primary cultures of human airway epithelial cells (HAE). MeV typically remains cell-associated in HAE and forms foci of infection, termed infectious centers, by directly spreading cell-to-cell.
View Article and Find Full Text PDFCCL4 Affects Eosinophil Survival via the Shedding of the MUC1 N-Terminal Domain in Airway Inflammation.
Cells
December 2024
Airway Disease Section, Department of Otorhinolaryngology, Kansai Medical University, Hirakata, Osaka 573-1010, Japan.
Eosinophilic chronic rhinosinusitis (ECRS), a CRS with nasal polyps (CRSwNP), is characterized by eosinophilic infiltration with type 2 inflammation and is highly associated with bronchial asthma. Intractable ECRS with poorly controlled asthma is recognized as a difficult-to-treat eosinophilic airway inflammation. Although eosinophils are activated and coincubation with airway epithelial cells prolongs their survival, the interaction mechanism between eosinophils and epithelial cells is unclear.
View Article and Find Full Text PDFEpigenetic Inhibitors Differentially Impact TGF-β1 Signaling Cascades in COPD Airway Smooth Muscle Cells.
Cells
December 2024
School of Life Science, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Chronic obstructive pulmonary disease (COPD) is characterized by progressive and incurable airflow obstruction and chronic inflammation. Both TGF-β1 and CXCL8 have been well described as fundamental to COPD progression. DNA methylation and histone acetylation, which are well-understood epigenetic mechanisms regulating gene expression, are associated with COPD progression.
View Article and Find Full Text PDFAssociation Between Chronic Obstructive Pulmonary Disease and Rheumatoid Arthritis: Findings of the Observational and Mendelian Randomization Study.
Int J Rheum Dis
January 2025
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Army Medical University, Chongqing, China.
Background: Airway inflammation is considered one of the pathogenic factors in rheumatoid arthritis (RA), but the role of chronic obstructive pulmonary disease (COPD) in the development of RA remains unclear. We used cross-sectional studies and Mendelian randomization (MR) analysis to explore the link between COPD and RA.
Methods: In National Health and Nutrition Examination Survey (NHANES) 2013-2018, the association between COPD and RA was investigated using weighted logistic regression models.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!