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A non-covalently bound redox indicator for electrochemical CRISPR-Cas12a and DNase I biosensors.
Anal Chim Acta
January 2025
Department of Chemistry and Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT, 84322, USA; Department of Chemistry, University of Louisiana at Lafayette, 300 East St. Mary Blvd, Lafayette, LA, 70504, USA. Electronic address:
A rapid and accurate biosensor for detecting disease biomarkers at point-of-care is essential for early disease diagnosis and preventing pandemics. CRISPR-Cas12a is a promising recognition element for DNA biosensors due to its programmability, specificity, and deoxyribonuclease activity initiated in the presence of a biomarker. The current electrochemical CRISPR-Cas12a-based biosensors utilize the single-stranded DNA (ssDNA) self-assembled on an electrode surface and covalently modified with the redox indicator, usually methylene blue (MB).
View Article and Find Full Text PDFFlexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids.
Biosensors (Basel)
December 2024
Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
In the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a redox indicator, fluorescent dye, or quencher for biosensing. To avoid this complex chemical modification, in this research, mismatched DNA with an intercalated metal ion complex (MIMIC) is employed as the probe sequence.
View Article and Find Full Text PDFDual-function paper-based biosensor for sensitive detection of hyaluronidase and human papillomavirus DNA using diffusion wet area as readout.
Biosens Bioelectron
March 2025
Institute for Advanced Study, Research Center for Differentiation and Development of TCM Basic Theory, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330004, China. Electronic address:
Herein, a novel dual-function paper-based biosensor using diffusion wet area as readout has been developed for simple and sensitive detection of hyaluronidase (HAase) and human papillomavirus (HPV) 16 DNA, respectively. The target-regulated-water absorption hydrogel synthesized by hyaluronic acid (HA) and single-stranded DNA (ssDNA) is chosen as an ideal material for diffusion wet area generation on paper. The hydrogel can be degraded through the enzymolysis of HA by HAase or the trans-cleavage of ssDNA by HPV DNA-activated CRISPR/cas12a system.
View Article and Find Full Text PDFQuencher-free CRISPR-based molecular detection using an amphiphilic DNA fluorescence probe.
Biosens Bioelectron
March 2025
Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, United States. Electronic address:
Rapid, sensitive, and specific nucleic acid detection methods play crucial roles in clinical diagnostics and healthcare. Here, we report a novel amphiphilic DNA fluorescence probe for CRISPR-based nucleic acid detection. Unlike conventional fluorophore-quencher probe detection system, our amphiphilic DNA fluorescence probe features a hydrophobic Cy5 fluorophore head and a hydrophilic single-stranded DNA (ssDNA) tail.
View Article and Find Full Text PDFDetection of HPV 16 and 18 L1 genes by a nucleic acid amplification-free electrochemical biosensor powered by CRISPR/Cas9.
Bioelectrochemistry
April 2025
School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea. Electronic address:
Article Synopsis
- - Cervical cancer is strongly associated with Human Papillomavirus (HPV) infection, making its detection crucial for women’s health, but traditional methods like RT-PCR can be expensive and slow.
- - This study presents a new electrochemical biosensor that uses CRISPR technology to detect HPV 16 and 18 genes without the need for nucleic acid amplification, enhancing both efficiency and cost-effectiveness.
- - The biosensor is highly sensitive, able to detect L1 genes at extremely low concentrations, and shows results comparable to conventional methods, indicating its potential for use in clinical diagnostics and point-of-care testing.
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