AI Article Synopsis

  • This study investigates the small RNA profiles in sperm to find unique miRNA patterns that may differentiate subfertile men from fertile men.
  • Utilizing Small RNA next-generation sequencing, researchers analyzed sperm from fertile men and validated findings with additional samples from subfertile and fertile men.
  • The results identified specific miRNAs with altered expressions in subfertile men, which correlate with key sperm characteristics, suggesting their potential use as biomarkers in fertility assessments.

Article Abstract

Purpose: To investigate small RNA profiles in sperm, identify stable miRNA patterns unique to sperm, and assess the behavior of consistently expressed miRNAs in sperm from subfertile men compared to fertile controls.

Methods: The small RNA profiles of single sperm from four proven fertile men were analyzed using Small RNA next-generation sequencing (NGS). Subsequently, a specific set of miRNAs was validated using RT-qPCR on additional sperm samples from 65 subfertile men from an infertility clinic and 30 proven fertile men.

Results: Small RNA sequencing revealed a diverse range of sperm small RNA biotypes, including miRNAs. The mapped read percentage ranged from 22.19% for single sperm to 83.29% for enriched sperm samples used at different RNA concentrations. In single sperm, a smaller proportion of sequences were attributed to piRNAs (2.79%), miRNA (0.94%), tRNA (0.82%), and rRNA (0.47%) compared to enriched sperm samples, where piRNA (41.68%), tRNA (20.31%), miRNA (11.11%), and rRNA (6.54%) were observed. Distinct detection rates and a higher number of detected miRNAs were noted with enriched sperm samples compared to single sperm obtained using either a micromanipulator or microdissection systems. Among the identified miRNAs, 110 were consistently present in all samples. RT-qPCR revealed 15 miRNAs with increased expression and 5 miRNAs with decreased expression in sperm samples from subfertile men compared to proven fertile men. These differentially validated miRNAs were significantly correlated, either positively or negatively, with sperm count, motility, and morphology.

Conclusion: The study extensively examines small RNAs in single sperm, identifying sperm-specific miRNAs that could serve as molecular markers to distinguish between subfertile and fertile men in clinical settings.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621271PMC
http://dx.doi.org/10.1007/s10815-024-03264-wDOI Listing

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