AI Article Synopsis
- The study introduces a sensitive and selective method for protein screening using surface-enhanced Raman scattering (SERS) with unique octahedral Au nanotrenches (OANTs), which are specialized nanoparticles designed to enhance light trapping.
- OANTs feature narrow trenches that improve electromagnetic field focusing, making them effective for detecting proteins at a very small scale (around 1 nm gap).
- The research demonstrated that this technique successfully distinguished His-tagged human serum albumin (HSA) from native HSA with 100% sensitivity and selectivity, showcasing the powerful combination of SERS and OANTs for advanced protein screening.
Article Abstract
This study represents a highly sensitive and selective approach to protein screening using surface-enhanced Raman scattering (SERS) facilitated by octahedral Au nanotrenches (OANTs). OANTs are a novel class of nanoparticles characterized by narrow, trench-like excavations indented into the eight facets of a Au octahedron. This unique configuration maximizes electromagnetic near-field focusing as the gap distance decreases to ∼1 nm. Owing to geometrical characteristics of the OANTs, near-field focusing can be maximized through the confinement and reflectance of light trapped within the trenches. We used Ni ions and molecular linkers to confer selective binding affinity for His-tagged proteins on the surfaces of the OANTs for SERS-based protein screening. Remarkably, SERS-based protein screening with the surface-modified OANTs yielded outstanding screening capabilities: 100% sensitivity and 100% selectivity in distinguishing His-tagged human serum albumin (HSA) from native HSA. This highlights the significantly enhanced protein screening capabilities achieved through the synergistic combination of SERS and the OANTs.
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| http://dx.doi.org/10.1021/acs.nanolett.4c03783 | DOI Listing |
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