N-Methyladenosine RNA Modification Regulates Maize Resistance to Maize Chlorotic Mottle Virus Infection.

J Agric Food Chem

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Published: October 2024

Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize. N-methyladenosine (mA) RNA modification has been proven to play important regulatory roles in plant development and stress response. In this study, we found that MCMV infection significantly up-regulated the mA level in maize, and methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were performed to investigate the distribution of mA modified peaks and gene expression patterns in MCMV-infected maize plants. The results showed that 1325 differentially methylated genes (DMGs) and 47 differentially methylated and expressed genes (DMEGs) were identified and analyzed. Moreover, the results of virus-induced gene silencing (VIGS) assays showed that and were required for MCMV infection, while silencing of , or significantly promoted MCMV infection in maize. Our findings provided novel insights into the regulatory roles of mA modification in maize response to MCMV infection.

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http://dx.doi.org/10.1021/acs.jafc.4c04832DOI Listing

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