positively regulates high-temperature tolerance of herbaceous peony by scavenging reactive oxygen species.

Unlabelled: Herbaceous peony ( Pall.) is a widely used famous traditional flower in China. It prefers cold and cool climate, but is not resistant to high temperature during summer in the middle and lower reaches of the Yangtze River. Previously, we found peroxidase (POD) is an important antioxidant enzyme that played an important role in high-temperature tolerance of . The present study isolated the candidate gene and verified its function in resisting high-temperature stress. And the results showed that had an open reading frame of 978 bp that encoded 325 amino acids. Its protein was localized to the cell membrane and cytoplasm. High-temperature stress induced expression. Heterologous overexpression of improved plant tolerance to high-temperature stress, decreased reactive oxygen species (ROS) accumulation, relative electrical conductivity and malondialdehyde content, and increased the ratio of variable fluorescence to highest fluorescence and POD activity. Conversely, silencing in could decrease POD activity, ROS scavenging capability and cell membrane stability when these plants were exposed to high-temperature stress. These results suggest that positively regulates high-temperature tolerance through ROS scavenging, which would provide a theoretical basis for improving high-temperature tolerance in .

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01505-x.