Background: Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.
Aim: The current study was carried out to identify if there is any presence of -related enterotoxin genes in cheese samples.
Methods: The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of , , , and enterotoxin genes in these samples.
Results: The conventional identification revealed the growth of . from the cheese samples. The PCR findings recorded the presence of the , , , and in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.
Conclusion: The current results indicate the presence of enterotoxin genes of in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.
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http://dx.doi.org/10.5455/OVJ.2024.v14.i8.36 | DOI Listing |
Gels
December 2024
Microbiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094 Bydgoszcz, Poland.
is a common etiological factor of hospital infections, which, in extreme cases, can lead to the death of patients. Most strains belonging to this bacterium species synthesize very dangerous toxins: toxin A (TcdA) and B (TcdB) and binary toxin (CDT). The aim of this study was to assess the suitability of agarose gel electrophoresis separation of multiplex PCR amplicons to investigate the toxinogenic potential of strains.
View Article and Find Full Text PDFJ Food Prot
December 2024
Université Paris-Saclay, Micalis Institute, INRAE, AgroParisTech, 78352 Jouy en Josas, France. Electronic address:
Staphylococcus aureus is a pathogenic microorganism often found in animal-derived foods and is known for its ability to readily develop resistance to antibiotic treatments. This study was designed to determine prevalence of S. aureus strains in raw milk and meat in Italy and to evaluate their antibiotic resistance profiles and biofilm production.
View Article and Find Full Text PDFWei Sheng Yan Jiu
November 2024
Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China.
Objective: To investigate the molecular typing characteristics, drug resistance status and drug resistance gene carrying of food-borne Staphylococcus aureus in Ningxia.
Methods: Staphylococcus aureus isolated from food safety risk monitoring project in Ningxia in the past ten years were collected, drug resistance was detected using microbroth dilution method, enterotoxins were detected by real-time PCR. The strains were genotyped by pulsed field gel electrophoresis(PFGE) using SmaI endonuclease.
Sci Rep
December 2024
Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, SAR, China.
The emergence of pandrug-resistant (PDR) and extensive drug-resistant (XDR) methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) isolates from bovine milk samples along with biofilm formation ability and harboring various virulence genes complicates the treatment of bovine mastitis and highlights the serious threat to public health. This study investigated for the first time the frequency, antimicrobial resistance profiles, biofilm-forming ability, virulence factors, spa and staphylococcal cassette chromosome mec (SCCmec) types of MRSA and VRSA isolated from clinical and subclinical bovine mastitis in Egypt. A total of 808 milk samples were collected from each quarter of 202 dairy animals, including 31 buffaloes and 171 cattle.
View Article and Find Full Text PDFmSphere
December 2024
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.
The toxigenic strains secrete tilymicin and tilivalline enterotoxins, which cause antibiotic-associated hemorrhagic colitis. Both enterotoxins are non-ribosomal peptides synthesized by enzymes encoded in two divergent operons clustered in a pathogenicity island. The transcriptional regulator Lrp (eucine-responsive egulatory rotein) controls the expression of several bacterial genes involved in virulence.
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