Background: The most common natural prion disease that primarily affects sheep and goats is scrapie. It belongs to a group of disorders known as transmissible spongiform encephalopathies, which impact both humans and animals.
Aim: The research is aimed to examine and confirm the presence of scrapie in Libya using immunohistochemistry (IHC) techniques.
Methods: Brain samples were collected from thirty-three sheep older than two years of age showing clinical signs resembling to scrapie during the period between 2018 and 2023, regardless of race or gender. Three animals, six months old, healthy, and without any symptoms, were used as negative controls. Different parts of the brain, including the obex and cerebellum, were taken from each case. The IHC technique used in this study involved staining with monoclonal antibody L42 and DAB (3,3'-diaminobenzidine) as a chromogenic substrate.
Results: The IHC examination showed the expression of prion proteins in brain tissue in twenty-three samples. The staining intensity was markedly observed in the neuronal cell bodies and around blood vessels.
Conclusion: The findings of this study provide evidence that scrapie exists in Libya.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415906 | PMC |
| http://dx.doi.org/10.5455/OVJ.2024.v14.i8.12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Predicted breeding values for relative scrapie susceptibility for genotyped and ungenotyped sheep.
Genet Sel Evol
December 2024
Faculty of Agricultural Sciences, Agricultural University of Iceland, 311, Borgarnes, Iceland.
Background: Scrapie is an infectious prion disease in sheep. Selective breeding for resistant genotypes of the prion protein gene (PRNP) is an effective way to prevent scrapie outbreaks. Genotyping all selection candidates in a population is expensive but existing pedigree records can help infer the probabilities of genotypes in relatives of genotyped animals.
View Article and Find Full Text PDFThe first meta-analysis of the G96S single nucleotide polymorphism (SNP) of the prion protein gene () with chronic wasting disease in white-tailed deer.
Front Vet Sci
November 2024
Department of Biological Sciences, Andong National University, Andong, Republic of Korea.
Background: Prion diseases are irreversible infectious neurodegenerative diseases caused by a contagious form of prion protein (PrP). Since chronic wasting disease (CWD)-infected white-tailed deer are strong carriers of the prion seed through corpses via scavenger animals, preemptive control based on genetic information for a culling system is necessary. However, the risk of CWD-related genetic variants has not been fully evaluated.
View Article and Find Full Text PDFActivation of IP10/CXCR3 Signaling is Highly Coincidental with PrP Deposition in the Brains of Scrapie-Infected Mice.
Biomed Environ Sci
November 2024
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory for Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 100084, Zhejiang, China;Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, Hubei, China;China Academy of Chinese Medical Sciences, Beijing 100700, China;Shanghai Institute of Infectious Disease and Biosafety, Shanghai 200003, China.
Objective: To analyze the relationship between Chemokine IP10 and its receptor CXCR3 during prion infection.
Methods: We investigated the increases in IP10 signals, primarily localized in neurons within the brains of scrapie-infected mice, using western blotting, ELISA, co-immunoprecipitation, immunohistochemistry, immunofluorescence assays, and RT-PCR.
Results: Both CXCR3 levels and activation were significantly higher in the brains of scrapie-infected mice and prion-infected SMB-S15 cells.
Roles of prion proteins in mammalian development.
Anim Cells Syst (Seoul)
December 2024
Department of Biotechnology, University of Rijeka, Rijeka, Croatia.
Prion protein (PrP) is highly conserved and is expressed in most tissues in a developmental stage-specific manner. Glycosylated cellular prion protein (PrP) is found in most cells and subcellular areas as a physiological regulating molecule. On the other hand, the amyloid form of PrP, scrapie PrP (PrP), causes transmissible pathogenesis in the central nervous system and induces degeneration of the nervous system.
View Article and Find Full Text PDFThis report presents the results of surveillance on transmissible spongiform encephalopathies in cattle, sheep, goats, cervids and other species, and genotyping in sheep and goats, carried out in 2023 by 27 Member States (MS, EU27), the United Kingdom (in respect of Northern Ireland, (XI)) and other eight non-EU reporting countries: Bosnia and Herzegovina, Iceland, Montenegro, North Macedonia, Norway, Serbia, Switzerland (the data reported by Switzerland include those of Liechtenstein) and Türkiye. In total, 948,165 cattle were tested by EU27 and XI (-3%, compared with 2022), with five atypical BSE cases reported (four H-type: two in Spain, one in France and one in Ireland; one L-type in the Netherlands); and 46,096 cattle by eight non-EU reporting countries with two atypical BSE cases reported by Switzerland. Three additional atypical BSE cases were reported by UK (1), USA (1) and Brazil (1).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!