Antiradical effect and phytochemical characterisation of the leaves of Benth.

The objective of this study was to isolate and identify compounds present in the leaves of Benth (Leguminosae-Mimosoideae) and evaluate the antiradical potential of the derived extracts and fractions. Seven compounds were successfully isolated from the ethanolic leaf extract, comprising gallic acid, flavonoids, triterpenoids, and phytosteroids. Structural identification was accomplished through comprehensive spectroscopic data analysis, including NMR and mass spectrometry (ESI-MS), and comparison with existing literature. The antiradical efficacy of both the extract and its fractions was determined through assays measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and quantifying total phenolics UV/Vis, revealing notable antioxidant activity in extracts from Benth, especially in the EtOH, EtOAc, and aqueous fractions, attributed to their high phenolic content and prevalence of flavonoids. These findings contribute to a deeper understanding of s chemical composition and its potential biological benefits.