AI Article Synopsis

  • The study investigates the effects of Kaixin Powder (KXP) on behavior and brain tissue in mice undergoing chemotherapy to understand how it may reduce cognitive impairment related to cancer treatment.
  • Thirty female BALB/c mice with breast cancer were divided into three groups for testing, including behavioral assessments and various pathological examinations to evaluate the impact of KXP compared to chemotherapy.
  • Results showed that KXP significantly improved behavior and cognition in mice, reduced neuroinflammation and cell death in the brain, and altered RNA expression profiles, suggesting its potential to mitigate cognitive damage during chemotherapy.

Article Abstract

The study aims to evaluate the effect of Kaixin Powder(KXP) on the behavior and brain tissue of chemotherapy-treated mice to explore its mechanism in alleviating chemotherapy-induced cognitive impairment in tumor-bearing mice. Thirty female BALB/c mice were inoculated with 4T1 breast cancer cells to establish a tumor-bearing mouse model and randomly divided into the tumor group, a doxorubicin group, and a KXP group. Behavioral tests, including open field test, elevated plus maze test, forced swimming test, tail suspension test, Morris water maze test, and novel object recognition test, were conducted. Pathological examinations, including hematoxylin-eosin staining, Nissl staining, toluidine blue staining, Fluoro-Jade B staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) assay, immunofluorescence staining, and transmission electron microscopy, were performed. Network pharmacology and whole transcriptome sequencing methods were used to analyze the mechanism of chemotherapy-induced cognitive impairment and the targets of KXP. The results showed that KXP prevented chemotherapy-induced behavioral changes(P<0.001), increased the total movement distance and central zone residence time in the open field test, increased exploration time in the open arm area in the elevated plus maze test, reduced immobility time in the forced swimming test and tail suspension test, reduced escape latency in the Morris water maze test and increased platform crossings, and improved cognitive index in the novel object recognition test. KXP also inhibited chemotherapy-induced neuroinflammation, apoptosis, and autophagy in the prefrontal cortex, and reshaped the RNA expression profile of the prefrontal cortex tissue during chemotherapy(P<0.05). In conclusion, KXP may alleviate chemotherapy-induced cognitive impairment in tumor-bearing mice by reshaping the RNA expression profile of prefrontal cortex tissue, thereby reducing neuronal tissue damage.

Download full-text PDF

Source
http://dx.doi.org/10.19540/j.cnki.cjcmm.20240430.401DOI Listing

Publication Analysis

Top Keywords

chemotherapy-induced cognitive
16
cognitive impairment
16
maze test
16
test
12
prefrontal cortex
12
breast cancer
8
impairment tumor-bearing
8
tumor-bearing mice
8
open field
8
field test
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!