AI Article Synopsis
- Human Enterovirus 71 (EV71) is a major cause of hand, foot and mouth disease (HFMD) worldwide, with an inactivated vaccine already available, but new advanced vaccine candidates are being explored.
- Researchers created two DNA-launched live attenuated vaccine candidates (pDL-EV71) that showed effectiveness in generating infectious EV71 in lab tests and were safe in animal models, with no clinical symptoms reported.
- Immunization with pDL-EV71 led to strong antibody responses, and a single dose provided complete protection against severe EV71 infection in newborn mice, indicating its potential as a viable vaccine candidate for further research.
Article Abstract
Human Enterovirus 71 (EV71) has emerged as one of the predominant causative agents of hand, foot and mouth disease (HFMD) with global impact. Despite the inactivated vaccine being licensed, other vaccine candidates based on advanced technology platforms are under development. In this report, we rationally designed and constructed two DNA-launched live attenuated vaccine candidates (pDL-EV71) under the control of specific promoters. In vitro and in vivo transfection with pDL-EV71 driven by the CMV promoter successfully yielded fully infectious EV71. More importantly, the administration of pDL-EV71 did not cause clinical symptoms following intracranial or intramuscular inoculation in neonatal and IFNα/βR mice, demonstrating its safety profile. Moreover, a single-dose or two-dose immunization with pDL-EV71 elicited robust neutralizing antibodies against EV71 as well as an antigen-specific cellular response in mice. A single-dose immunization with 10 μg of pDL-EV71 conferred complete protection against lethal EV71 infection in neonates born to immunized maternal mice. Overall, our present results demonstrate that pDL-EV71 is a safe and effective vaccine candidate against EV71 for further development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738765 | PMC |
| http://dx.doi.org/10.1016/j.virs.2024.09.008 | DOI Listing |
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